| PSP94融合蛋白在大肠杆菌中的表达及其抗前列腺癌活性分析 |
| |
| 引用本文： | 刘建香,刘丽,刘立忠,王颖,谢宝树,冷爱军,苏旭,刘树铮.PSP94融合蛋白在大肠杆菌中的表达及其抗前列腺癌活性分析[J].中国生物工程杂志,2000,20(1):8-12. |
| |
| 作者姓名： | 刘建香 刘丽 刘立忠 王颖 谢宝树 冷爱军 苏旭 刘树铮 |
| |
| 作者单位： | 1. 白求恩医科大学卫生部重点实验室放射生物 130021 长春;
2. 空军总医院空军分子生物学研究中心 100036 北京 |
| |
| 摘 要： | 在从成年人正常前列腺组织中获得人９４个氨基酸的前列腺分泌蛋白（ＰＳＰ９４）ｃＤＮＡ基础上，利用ＰＬ表达系统，实现了人ＰＳＰ９４成熟肽Ｎ 末端带有１９个外源氨基酸的融合蛋白在大肠杆菌中的表达。目的蛋白在细胞中主要以包涵体形式存在，表达量约占菌体总蛋白的３０％，分子量约为１６－５ｋＤ。表达产物在人前列腺癌细胞ＰＣ ３上活性分析表明，该融合蛋白能明显抑制前列腺癌细胞的生长。
|
| 关 键 词： | 94个氨基酸的前列腺分泌蛋白 融合蛋白 表达 抗前列腺癌 |
Expression of Human Prostate Secretory Protein of 94 Amino Acids Fusion Protein in E.coli and Activity Assay of the Expressed Product |
| |
| Liu Jianxiang\ \ Liu Li\ Liu Lizhong MH Radiobiology,Norman Bethune University of Medical Sciences,Changchun\ Research Center of Molecular Biology,Airforce Hospital,Beijing.Expression of Human Prostate Secretory Protein of 94 Amino Acids Fusion Protein in E.coli and Activity Assay of the Expressed Product[J].China Biotechnology,2000,20(1):8-12. |
| |
| Authors: | Liu Jianxiang\ \ Liu Li\ Liu Lizhong MH Radiobiology Norman Bethune University of Medical Sciences Changchun\ Research Center of Molecular Biology Airforce Hospital Beijing |
| |
| Institution: | Liu Jianxiang\ *\ Liu Li\ Liu Lizhong *MH Radiobiology,Norman Bethune University of Medical Sciences,Changchun\ 130021 Research Center of Molecular Biology,Airforce Hospital,Beijing 100036 |
| |
| Abstract: | We had previously cloned the whole length cDNA of human prostate secretory protein of 94 amino acids (PSP94) from normal adult prostatic tissues by RT\|PCR.In this study, we reported expression of human PSP94 fusion protein in E.coli.The chimeric gene coding for 19aa\|PSP94 fusion proteins under control of the P\-L promoter was constructed. The expression was assayed by SDS\|PAGE,which showed that the molecular weight of the 19aa\|PSP94 fusion protein was in accordance with the estimated value of 16.5kD.It was 30% of total bacterial protein and in the form of inclusion body. Through biological activity analysis, 19aa\|PSP94 fusion protein has anti\|prostatic cancer activity. |
| |
| Keywords: | Prostate Secretory Protein of 94 amino acids (PSP94) Fusion protein Expression Anti\|prostatic cancer |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《中国生物工程杂志》浏览原始摘要信息 |
| 点击此处可从《中国生物工程杂志》下载免费的PDF全文 |
