RNA degrading activity is tightly associated with the multicatalytic proteinase, ingensin |
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| Authors: | T Tsukahara K Tanaka T Ogawa S Ishiura R Funabiki H Sugita |
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| Institution: | National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan. |
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| Abstract: | The multicatalytic proteinase, ingensin, was purified to homogeneity from chicken liver. rRNA-degrading activity was co-eluted with the purified multicatalytic proteinase from a TSK-3000SW column. This RNA-degrading activity was inactivated by heat treatment and the addition of a low concentration of SDS. Therefore, the RNA-degrading activity co-eluted with the multicatalytic proteinase was not due to contamination by low-molecular-mass RNases. These results strongly suggest that this RNA-degrading activity was tightly associated with the multicatalytic proteinase, ingensin. |
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