Sequence and Gene Expression Analyses of Plasmid pHPM8 from Helicobacter pylori Reveal the Presence of Two Operons with Putative Roles in Plasmid Replication and Antibiotic Activity |
| |
| Authors: | Mariam Qui ones, John E. Knesek,Sarah A. McIntire |
| |
| Affiliation: | Biology Department, Texas Woman's University, Denton, Texas 76204, USA. |
| |
| Abstract: | The DNA sequence of a 7.8-kb Helicobacter pylori plasmid, pHPM8, was determined. Six open reading frames (ORFs) were present. Ribonuclease protection studies showed that ORF1/ORF2 and ORF3/ORF4 genes are organized in operons possibly involved in plasmid replication and in production of a peptide with antibiotic activity, respectively. Finding areas of pHPM8 with a high level of identity to H. pylori chromosomal DNA supported the hypothesis that recombination occurs between plasmids and the chromosome of H. pylori. |
| |
| Keywords: | Helicobacter plasmid DNA sequence. |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
