Construction of a representative genomic library from a hexaploid

The large genome size and the great amount of DNA repeats make it rather difficult to obtain a representative hexaploid wheat genomic library. The protocol is given with modifications to phage isolation and to purification of vector and plant DNAs by electrogenomic libraries of the soft wheat variety carrying a translocated rye chromosome arm 1RS were constructed in arecD E. coli strain to reduce recombinational loss.