| 以魔芋葡甘聚糖凝胶为载体亲和层析分离纯化猪血SOD |
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| 引用本文： | 周立,郑远旗,李建吾,蒋磊,贾成禹.以魔芋葡甘聚糖凝胶为载体亲和层析分离纯化猪血SOD[J].中国生物化学与分子生物学报,1996,12(3):344-347. |
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| 作者姓名： | 周立 郑远旗 李建吾 蒋磊 贾成禹 |
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| 作者单位： | 四川大学生物系，中国科学院成都生物所 |
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| 摘 要： | 以魔芋葡甘聚糖（ＫＧＭ）凝胶作为铜金属螯合亲和层析的载体一步亲和纯化猪血ＳＯＤ，得到电泳均一，比活为８６２２Ｕ／ｍｇ，纯化倍数为７７．８倍的ＳＯＤ，其回收率为８５．４％．探讨了魔芋葡甘聚糖凝胶作为亲和载体的可能性及前景．
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| 关 键 词： | 魔芋葡甘聚糖凝胶 超氧化物歧化酶 金属螯合亲和层析 |
| 收稿时间： | 1996-06-20 |
Purification of Porcine SOD by Metal Chelate Affinity Chromatography Using KGM Gel as Substrate |
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| Zhou Li,Zheng Yuan-Qi,Li Jian-Wu,Jiang Lei.Purification of Porcine SOD by Metal Chelate Affinity Chromatography Using KGM Gel as Substrate[J].Chinese Journal of Biochemistry and Molecular Biology,1996,12(3):344-347. |
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| Authors: | Zhou Li Zheng Yuan-Qi Li Jian-Wu Jiang Lei |
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| Institution: | (Biology Department of Sichuan University, Chengdu 610064)Jia Cheng-Yu(Chengdu Institute of Biology, Academia Sinica, Chengdu 610041 |
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| Abstract: | KGM gel was used as substrate for chromatography,epichlorohydrin as activitor and iminodiacetic acid as ligands, then Cu was chelated to form Cu-metal chelate affinty chromatographic column. The SOD in procine red cells was purified to homogeneous in one step affinity chromatography. Its specfic activity is 8622 U/mg, yield is 85. 4% and the purification factor is 77. 8. It was discussed that it is possible that Sephadex G-200 could be substituted by KGM gel in biological macromolecule investigation. |
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| Keywords: | KGM gel Superoxide dismutase Metal chelate affinity chromatograph |
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