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A simple PCR/RFLP analysis can differentiate between Candida albicans, Aspergillus niger, and Aspergillus fumigatus
Authors:Nilgun Isik  Lewis White  Rosemary Barnes  Christopher J. Poynton  Ken I. Mills
Affiliation:(1) Department of Haematology, University of Wales College of Medicine, CF14 4XN Cardiff, Wales, UK;(2) Department of Microbiology, University of Wales College of Medicine, CF14 4XN Cardiff, Wales, UK
Abstract:
The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish between Candida and Aspergillus species and to classify Aspergillus strains.
Keywords:Candida    Aspergillus   markers
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