| 鲫鱼不同组织过氧化氢酶同工酶活性的比较 |
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| 引用本文： | 许黎黎,邵雪玲. 鲫鱼不同组织过氧化氢酶同工酶活性的比较[J]. 氨基酸和生物资源, 2003, 25(4): 69-71. DOI: 10.3969/j.issn.1006-8376.2003.04.020 |
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| 作者姓名： | 许黎黎 邵雪玲 |
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| 作者单位： | 武汉大学生命科学学院,武汉,430072;武汉大学生命科学学院,武汉,430072 |
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| 基金项目： | 实验教学中心资助项目;; |
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| 摘 要： | 对鲫鱼的心、鳃、肉、卵四种组织进行聚丙烯酰胺凝胶电泳并用KMnO4 法染色 (定性 ) ,同时与FeCl3-K3Fe(CN) 6 系统法进行对照 ,发现两种染色法得到的酶带具有一致性。然后用钼酸铵比色法测定四种组织中过氧化氢酶的活性 (定量 ) ,发现四种组织中过氧化氢酶的活性大小依次为 :心 >卵 >鳃 >肉。
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| 关 键 词： | 过氧化氢酶 同工酶 活性染色比较 钼酸铵比色法定量 |
| 文章编号： | 1006-8376(2003)04-0069-02 |
| 修稿时间： | 2003-09-12 |
Comparison of Activities of Isozyme Family Members of the Catalase in Different Tissues of Crucian |
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| XU Li-li,SHAO Xue-ling. Comparison of Activities of Isozyme Family Members of the Catalase in Different Tissues of Crucian[J]. Amino Acids & Biotic Resources, 2003, 25(4): 69-71. DOI: 10.3969/j.issn.1006-8376.2003.04.020 |
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| Authors: | XU Li-li SHAO Xue-ling |
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| Abstract: | The purified tissues of heart, gill, meat and roe of crucian were run elechophoresis on polyacrylamide gel and stained with KMnO 4 (qualitative). At the same time,staining method with FeCl 3-K 3Fe(CN) 6 was done and found out that both of the achromatic bands of the isozymes had consistency. Then the activities of these four isozymes were measured using ammonium molybdate colorimetry (quantitative). The activities of the isolymes in these four tissues were: heart>roe>gill>meat. |
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| Keywords: | catalase isozyme staining molybdate colorimetry |
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