Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions. |
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| Authors: | Takeshi Akao Motoaki Sano Osamu Yamada Terumi Akeno Kaoru Fujii Kuniyasu Goto Sumiko Ohashi-Kunihiro Kumiko Takase Makoto Yasukawa-Watanabe Kanako Yamaguchi Yoko Kurihara Jun-ichi Maruyama Praveen Rao Juvvadi Akimitsu Tanaka Yoji Hata Yasuji Koyama Shotaro Yamaguchi Noriyuki Kitamoto Katsuya Gomi Keietsu Abe Michio Takeuchi Tetsuo Kobayashi Hiroyuki Horiuchi Katsuhiko Kitamoto Yutaka Kashiwagi Masayuki Machida Osamu Akita |
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| Institution: | National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan. akao_t@nrib.go.jp |
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| Abstract: | We performed random sequencing of cDNAs from nine biologically or industrially important cultures of the industrially valuable fungus Aspergillus oryzae to obtain expressed sequence tags (ESTs). Consequently, 21 446 raw ESTs were accumulated and subsequently assembled to 7589 non-redundant consensus sequences (contigs). Among all contigs, 5491 (72.4%) were derived from only a particular culture. These included 4735 (62.4%) singletons, i.e. lone ESTs overlapping with no others. These data showed that consideration of culture grown under various conditions as cDNA sources enabled efficient collection of ESTs. BLAST searches against the public databases showed that 2953 (38.9%) of the EST contigs showed significant similarities to deposited sequences with known functions, 793 (10.5%) were similar to hypothetical proteins, and the remaining 3843 (50.6%) showed no significant similarity to sequences in the databases. Culture-specific contigs were extracted on the basis of the EST frequency normalized by the total number for each culture condition. In addition, contig sequences were compared with sequence sets in eukaryotic orthologous groups (KOGs), and classified into the KOG functional categories. |
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