Cloning vector system forNocardia spp. |
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| Authors: | Yao Wensheng Yang Yunliu Chiao Juishen |
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| Institution: | (1) Shanghai Institute of Plant Physiology, Chinese Academy, 200032 Shanghai, P.R. China;(2) Present address: Department of Biological Sciences, State University of New York at Buffalo, 14260 Buffalo, NY, USA |
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| Abstract: | An electro-transformation system has been developed forNocardia asteroides andNocardia corallina by using aNocardia-Escherichia coli shuttle vector. The shuttle vector, named pCY104, was constructed by joining a 2.5-kb crypticN. asteroides plasmid pCY101 with theE. coli plasmid pIJ4625. The resistance genes for kanamycin, chloramphenicol, and thiostrepton on plasmid pCY104 were expressed inN. asteroides andN. corallina. The transformation method was optimized forN. asteroides, and transformation efficiency of 8×104 transformants per  g plasmid DNA was achieved routinely. |
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