不同栽培基质对糙皮侧耳不同发酵方式下产漆酶活性的影响

为了比较糙皮侧耳栽培种在不同常规栽培基质上的漆酶活性,分析更适合糙皮侧耳生长的栽培基质,以1株糙皮侧耳Pleurotus ostreatus栽培菌株为研究材料,研究在固态和液态发酵条件下添加木屑、玉米芯和棉籽壳这3种栽培基质后,对其产漆酶活性的影响。结果表明,不同栽培基质对糙皮侧耳漆酶活性具有极显著的影响（P<0.001）;不同发酵方法对糙皮侧耳漆酶活性也具有极显著的影响（P<0.001）,仅第2天差异不显著。固体发酵与液体发酵条件下,糙皮侧耳在棉籽壳培养基上所检测到的漆酶活性均高于在木屑或者玉米芯培养基上,表明棉籽壳对提高糙皮侧耳漆酶活性的诱导能力更强。此外,糙皮侧耳在棉籽壳培养基上能够快速分泌漆酶,表明棉籽壳对缩短糙皮侧耳漆酶分泌时间的诱导能力更强。

Effects of different culture substrate on laccase activities of Pleurotus ostreatus under different fermentation conditions

A comparative analysis was made between laccase activities of Pleurotus ostreatus cultivated on different conventional substrate, i.e. sawdust, corncob, and cottonseed hull, under solid-state and submerged fermentation conditions for the purposes of selecting most suitable cultivation substrate. The results demonstrated that various culture substrate remarkably affected the laccase activities of P. ostreatus (P<0.001). The laccase activities of P. ostreatus under different fermentation methods were significantly different (P<0.001), but no difference was found on the 2nd day of cultivation (P>0.05). Under the conditions of solid-state fermentation and submerged fermentation, the laccase activity detected in synthetic medium containing cottonseed hull was higher than that in synthetic medium containing sawdust or corncob, indicating that the cottonseed hull could be considered as a better inducer for enhancing the laccase activities of P. ostreatus. The synthetic medium containing cottonseed hull was in favour of speeding up the laccase secretion from P. ostreatus, and advancing the time of enzyme production.