Plasmalemma hyperpolarity and culture of sense and antisense abp transgenic tobacco protoplasts |
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Authors: | NI Di an WANG Lingjian XU Zhihong XIA Zhen'ao |
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Institution: | (1) Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, 200032 Shanghai, China;(2) Chinese Academy of Sciences, 100864 Beijing, China |
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Abstract: | The relationship among transfer and expression of auxin binding protein gene (abp), auxin (NAA)-induced plasmalemma hyperpolarity and sensibility to auxin during protoplast culture was studied by measuring
transmembrane potential difference (Em) and culturing the protoplasts of sense and antisenseabp transgenic tobacco. The concentration of NAA inducing the highest degree of hyperpolarity of senseabp transgenic tobacco protoplasts was lower than the control, and in protoplast culture, their sensibility to auxin increased.
The concentration of antisenseabp transgenic tobacco protoplasts was higher than the control, and in protoplast culture, their sensibility to auxin decreased.
These results demonstrated that ABP synthesized in endoplasmic reticulum needed to transport to cell membrane and functioned
there.
Project supported by the State Key Laboratory of Plant Molecular Genetics and National Natural Science Foundation of China
(Grant No. 39670078). |
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Keywords: | auxin auxin binding protein plasmalemma hyperpolarity protoplast culture transgenic tobacco transmembrane potential difference |
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