In vitro evaluation of the antioxidant activity of calcium fructoborate |
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Authors: | R Scorei V M Cimpoiasu Dana Iordachescu |
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Institution: | (1) Department of Biochemistry, University of Craiova, 13 A.I. Cuza, 1100 Craiova, Romania;(2) Research Center for Biochemistry and Molecular Biology, University of Bucharest, 93-95 Spl. Independentei, Bucharest, Romania |
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Abstract: | Although increasing evidence shows the nutritional benefits of calcium fructoborate (CF) on animals and humans, its action
mechanism has not been clearly identified. The present study aims to investigate the possible antioxidant function of CF.
Based on its efficiency in skin wound healing, the authors tested whether CF possesses antioxidant properties on human keratinocytes
cultures, in a complete serum-free medium (KMK-2; Sigma). The cells treated with CF (0–450 nmol/culture medium) were exposed
to exogenous 100 μmol of hydrogen peroxide to mimic the oxidative stress. The changes in general cell oxidant production evaluated
with dihydrorhodamine-123 showed that the intracellular reactive oxygen species (ROS) were markedly reduced by preincubation
with CF. The maximum antioxidant activity was notice at 90 nmol CF. To assess the reactivity of CF on ROS, we analyzed its
ability to inhibit the superoxide-dependent auto-oxidation of pyrogallol. The CF inhibited the pyrogallol auto-oxidation depending
on time and concentration, which suggests its possible role as a superoxide radical scavenger. Taken together, our results
indicate that CF has antioxidant activity, which could have clinical significance in protecting cells from oxidant-induced
injury. A hypothetic mechanism for the antioxidant activity of CF is proposed. |
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Keywords: | Calcium fructoborate (CF) antioxidant activity reactive oxygen species (ROS) superoxide radicals superoxide dismutase (SOD) keratinocytes |
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