Characterisation of Kir2.0 proteins in the rat cerebellum and hippocampus by polyclonal antibodies |
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| Authors: | A H Stonehouse J H Pringle R I Norman P R Stanfield E C Conley W J Brammar |
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| Institution: | (1) Department of Biochemistry, Adrian Building, University of Leicester, Leicester, LE1 7RH, UK Tel.: +44-116-2525586, Fax: +44-116-2525616, GB;(2) Department of Pathology, Robert Kilpatrick Building, University of Leicester, Leicester, LE2 7LX, UK, GB;(3) Department of Medicine, Robert Kilpatrick Building, University of Leicester, Leicester, LE2 7LX, UK, GB;(4) Department of Cell Physiology and Pharmacology, Maurice Shock Building, University of Leicester, Leicester, LE1 9HN, UK, GB;(5) Department of Pathology, c/o The Centre for Mechanisms of Human Toxicity, Hodgkin Building, Lancaster Road, Leicester, LE1 9HN, UK, GB |
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| Abstract: | Rabbit polyclonal antibodies were raised to rat Kir2.0 (Kir2.1, Kir2.2 and Kir2.3) inwardly rectifying potassium ion channel
proteins. The antibody specificities were confirmed by immunoprecipitation of 35S]-methionine-labelled in vitro translated channel proteins and western blotting. Immunohistochemistry revealed a different
patterns of expression of Kir2.0 subfamily proteins in the rat hind-brain (cerebellum and medulla) and fore-brain (hippocampus).
Notably, only Kir2.2 protein was detected in the cerebellum and medulla, Kir2.1, Kir2.2 and Kir2.3 proteins were expressed
in the hippocampus and immunostaining was not limited to neuronal cell types. Anti-Kir2.1 (fore-brain only) and anti-Kir2.2
(fore- and hind-brain) antibodies showed positive staining in macroglia, endothelia, ependyma and vascular smooth muscle cells.
In contrast, anti-Kir2.3 (fore-brain only) immunostaining was limited to neurons, macroglia and vascular smooth muscle. These
results indicate that specific regions within the rat fore- and hind-brain have differential distributions of inwardly rectifying
potassium ion channel proteins.
Accepted: 12 October 1999 |
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