Hemagglutinating activity and motility of the bacterium <Emphasis Type="Italic">Azospirillum brasilense</Emphasis> in the presence of various nitrogen sources |
| |
Authors: | A V Shelud’ko E G Ponomareva O E Varshalomidze E P Vetchinkina E I Katsy V E Nikitina |
| |
Institution: | 1.Institute of Biochemistry and Physiology of Plants and Microorganisms,Russian Academy of Sciences,Saratov,Russia |
| |
Abstract: | Hemagglutinating activity of the Azospirillum brasilense strain Sp245 grown in liquid media and the swarming motility of those bacteria grown in semisolid media vary significantly
depending on the nitrogen source. In media with nitrate or nitrite, an increase in the hemagglutinating activity and a decrease
in the swarming circles’ diameter of Sp245 were observed, compared to bacteria grown in the presence of ammonium or N2. A ∼67-kDa hemagglutinin exhibiting affinity to the O-specific polysaccharide, an acidic D-rhamnan (OPS-I), was isolated
from the surface of Sp245 cells. Introduction of the hemagglutinin into the media resulted in a decrease in the Sp245 cell
motility while not affecting its mutants lacking the acidic D-rhamnan or the Sp245.5 mutant with a different OPS structure.
Cells of strain Sp245.5 demonstrated hemagglutinating activity two times higher than that of the parent Sp245 strain and formed
“diffuse” colonies, rather than distinct swarming circles Sp245 formed when grown in a semisolid medium. The data obtained
demonstrate that intercellular contacts mediated by the interaction between the surface hemagglutinin and OPS-I, which is
sensitive to environmental factors, affect the collective motility of cells. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|