Hereditary spherocytosis of man. Altered binding of cytoskeletal components to the erythrocyte membrane |
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| Authors: | John S Hill William H Sawyer Geoffrey J Howlett and James S Wiley |
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| Institution: | Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria 3052, Australia;Department of Haematology, Austin Hospital, Heidelberg, Victoria 3085, Australia |
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| Abstract: | The endogenous respiration of the rumen ciliate Dasytricha ruminantium maintained under an O2 tension of 2kPa (approximately 0.02 atm) was partially inhibited by KCN (40% inhibition) and NaN3 (58% inhibition). The organisms lack cytochromes, and sensitivity of respiration to KCN, NaN3, chloroquine and quercetin suggest that the operation of flavoprotein-iron-sulphur-mediated electron transport. As in Tritrichomonas foetus, hydrogenosomal respiration can be stimulated by the addition of CoA in the presence of 0.025% Triton X-100; stimulation by ADP was not detected. Stimulation of pyruvate-supported O2 uptake by Pi suggests that acetate is produced via acetyl phosphate. |
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