| Gene controlled by promoter--P_(TH4) depending on whiG of Streptomyces coelicolor |
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| 作者姓名： | 谭华荣 杨海花 田宇清 吴畏 董可宁 K.F.Chater |
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| 作者单位： | Institute of Microbiology，Chinese Academy of Sciences，Beijing 100080，China，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100080，China，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100080，China，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100080，China，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100080，China，John Innes Institute，Norwich，England |
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| 基金项目： | Project supported by Chinese Academy of Sciences and the Royal Society of UK. |
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| 摘 要： | The downstream gene controlled by promoter--PTH4 which is related to Streptomycesdifferentiation was cloned, and its sequence was determined by the dideoxy chain termination method. The results indicated that the 1597 bp of DNA fragment conferred a complete open reading frame (ORF). In searches of databases, the deduced product of the ORF was not homologous with any known proteins; it may be a new protein. The function of the gene was studied using the strategy of gene disruption; the actinorhodin could not be produced when this gene was disrupted. Therefore, this gene may be related to actinorhodin biosynthesis in Streptomyces coelicolor, and the result also shows that this gene may play a role in multiple level regulation of differentiation genes in Streptomyces.
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Gene controlled by promoter--P_(TH4) depending on whiG of Streptomyces coelicolor |
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| TAN Huarong YANG Haihua,TIAN YuqingWU Wei,DONG Kening.Gene controlled by promoter--P_(TH4) depending on whiG of Streptomyces coelicolor[J].Science China Life sciences,1996(6). |
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| Authors: | TAN Huarong YANG Haihua TIAN YuqingWU Wei DONG Kening |
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| Abstract: | The downstream gene controlled by promoter--PTH4 which is related to Streptomycesdifferentiation was cloned, and its sequence was determined by the dideoxy chain termination method. The results indicated that the 1597 bp of DNA fragment conferred a complete open reading frame (ORF). In searches of databases, the deduced product of the ORF was not homologous with any known proteins; it may be a new protein. The function of the gene was studied using the strategy of gene disruption; the actinorhodin could not be produced when this gene was disrupted. Therefore, this gene may be related to actinorhodin biosynthesis in Streptomyces coelicolor, and the result also shows that this gene may play a role in multiple level regulation of differentiation genes in Streptomyces. |
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| Keywords: | Streptomyces differentiation regulation promoter gene disruption |
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