α2-macroglobulin ‘fast’ forms inhibit superoxide production by activated macrophages |
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Authors: | Maureane Hoffman Steven R Feldman Salvatore V Pizzo |
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Institution: | The Department of Pathology and Biochemistry, Duke University medical Center, Durham, NC 27710, U.S.A. |
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Abstract: | Mouse peritoneal macrophages activated by bacillus Calmette-Guerin (BCG) were incubated with human α2-macroglobulin converted to its ‘fast’ form with either trypsin or methylamine before being stimulated with phorbol myrystate acetate. Both α2-macroglobulin-trypsin and α2-macroglobulin-methylamine inhibited macrophage production of superoxide anion (O2−) while native α2-macroglobulin had little effect except at high concentration. The α2-macroglobulin ‘fast’ forms, which bind with a Kd of about 8 nM, inhibited 50% generation of O2−(ID50) at a concentration of 7 nM while α2-macroglobulin inhibited O2− production with an ID50 of 141 nM. The ‘fast’ forms of α2-macroglobulin may play a role in the feedback regulation of inflammatory reactions. |
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Keywords: | α 2-macroglobulin Macrophage Superoxide production (Murine peritoneum) |
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