Genetic analysis of two Portuguese populations of <Emphasis Type="Italic">Ruditapes decussatus</Emphasis> by RAPD profiling |
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Authors: | Jorge C Pereira Raquel Chaves Alexandra Leitão Domitilia Matias Henrique Guedes-Pinto |
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Institution: | 1.Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology,University of Trás-os-Montes and Alto Douro, IBB-CGB/UTAD,Vila Real,Portugal;2.Department of Genetics and Biotechnology,UTAD,Vila Real,Portugal;3.Instituto Nacional de Recursos Biológicos (INRB/L-IPIMAR),Olh?o,Portugal |
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Abstract: | The clam Ruditapes decussatus is commercially important in the south of Portugal. The random amplified polymorphic DNA (RAPD) technique was applied to
assess the genetic diversity and population structure of two Portuguese populations occurring in the Ria Formosa (Faro) and
the Ria de Alvor, respectively. Twenty-five individuals of each population were investigated by RAPD profiles. Genetic diversity
within populations, measured by the percentage of polymorphic loci (%P), varied between 68.57% (Alvor) and 73.88% (Faro). Shannon’s information index (H) and Nei’s gene diversity (h) were 0.281 and 0.176, respectively, for the Alvor population and 0.356 and 0.234 for the Faro population. Overall, genetic
variation within R. decussatus populations was high. The total genetic diversity (H
T) was explained by a low variation between populations (G
ST = 0.145), which is consistent with high gene flow (N
m = 2.9). The analysis of molecular variance (AMOVA) showed that 65% of variability is within populations and 35% between populations
(ΦPT = 0.345; P ≥ 0.001). The value of Nei’s genetic distance was 0.0881, showing a low degree of population genetic distance, despite the
different geographic origin. This is the first study on the population genetics of R. decussatus by RAPD technique. The results may be useful for restocking programs and aquaculture. |
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