Soft rot inciting <Emphasis Type="Italic">Pectobacterium carotovorum</Emphasis> (syn. <Emphasis Type="Italic">Erwinia carotovora</Emphasis>) is unlikely to be transmitted as a latent pathogen in micropropagated banana |
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Authors: | Pious Thomas Chinnaian Goplakrishnan Manem Krishnareddy |
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Institution: | (1) Division of Biotechnology, Indian Institute of Horticultural Research, Hessarghatta Lake Post, Bangalore, 560089, India;(2) Division of Plant Pathology, Indian Institute of Horticultural Research, Hessarghatta Lake Post, Bangalore, 560089, India |
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Abstract: | This study was undertaken to ascertain if the soft rot inciting Pectobacterium carotovorum/Erwinia carotovora would pass through the micropropagated bananas as a latent pathogen and cause disease during or post acclimatization. In
vitro cultures of ‘Grand Naine’ were exposed to the pathogen by providing 100 μl of inoculum (0.001–1.0 at OD600 nm) at the lower leaf axil. These cultures showed a gradual development of soft rot symptoms coupled with obvious bacterial
colony growth on banana proliferation medium and consequent plant mortality within a month irrespective of the inoculum level
employed. Plants carried forward to acclimatization following inoculation in vitro failed to establish ex vitro. Monitoring
the normal field-grown suckers at culture initiation through PCR screening employing soft rot Erwinia primers did not show the amplification of the 119-bp fragment as seen with the pure cultures of pathogen. Further testing
of micropropagated banana plants through soil inoculation, in vitro culturing and PCR screening ruled out the possibility
of the pathogen surviving in micropropagated stocks in latent form as the organism outgrew and killed the cultures. It emerged
that the infection possibly takes place in the nursery. This information will be of particular value for the plant tissue
culture industry, plant pathologists and quarantine agencies. |
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