Improved growth and acclimatization of somatic embryo-derived Oplopanax elatus plantlets by ventilated photoautotrophic culture |
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Authors: | S Y Park H K Moon H N Murthy and Y W Kim |
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Institution: | (1) Dpto. B.O.S. C/ Catedrático Rodrigo Uría, s/n, Lab. Fisiología Vegetal, 33071 Oviedo, Asturias, Spain;(2) 46113 Montcada de l'Horta, València, Spain;(3) 33071, Oviedo, Asturias, Spain |
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Abstract: | To improve large-scale in vitro production of Oplopanax elatus Nakai, we cultured somatic embryo-derived plantlets under a heterotrophic condition (semi-solid culture with sucrose), photoautotrophic
condition (semi-solid culture without sucrose), or modified photoautotrophic condition (liquid culture with forced ventilation).
The plantlets grown under the modified photoautotrophic condition had more leaves as well as higher chlorophyll content, and
higher net photosynthetic rate than those grown under the conventional conditions. Further, the photoautotrophically grown
plantlets acclimatized better and sooner upon ex vitro transplantation than did the conventionally cultured plantlets. Consequently, a photoautotrophic culture method with forced
ventilation is effective for enhancing the growth and acclimatization of O. elatus. |
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