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Transgenic rice plants expressing trichothecene 3-<Emphasis Type="Italic">O</Emphasis>-acetyltransferase show resistance to the <Emphasis Type="Italic">Fusarium</Emphasis> phytotoxin deoxynivalenol |
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| Authors: | Shuichi Ohsato Tetsuko Ochiai-Fukuda Takumi Nishiuchi Naoko Takahashi-Ando Shinzo Koizumi Hiroshi Hamamoto Toshiaki Kudo Isamu Yamaguchi Makoto Kimura |
| Institution: | (1) Plant & Microbial Metabolic Engineering Research Unit and Laboratory for Remediation Research, Discovery Research Institute (DRI) and Plant Science Center (PSC1), RIKEN, 2-1 Hirosawa, Wako Saitama, 351-0198, Japan;(2) Division of Functional Genomics, Advanced Science Research Center, Kanazawa University, 13-1 Takaramachi, Kanazawa Ishikawa, 920-0934, Japan;(3) Department of Plant Pathology, National Agricultural Research Center (NARC), Kannondai 3-1-1, Tsukuba Ibaraki, 305-8666, Japan;(4) Laboratory for Adaptation and Resistance, Plant Science Center (PSC1), RIKEN, Suehiro 1-7-22, Tsurumi, Yokohama Kanagawa, 230-0045, Japan;(5) Environmental Molecular Biology Laboratory, Discovery Research Institute (DRI), RIKEN, 2-1 Hirosawa, Wako Saitama, 351-0198, Japan;(6) Present address: Shibata Distinguished Senior Scientist Laboratory, Discovery Research Institute (DRI), RIKEN, 2-1 Hirosawa, Wako Saitama, 351-0198, Japan;(7) Present address: Laboratory for Neural Cell Polarity, Brain Science Institute (BSI), RIKEN, 2-1 Hirosawa, Wako Saitama, 351-0198, Japan;(8) Present address: Presidential Office, Agricultural Chemicals Inspection Station (ACIS), 2-772 Suzuki-cho, Kodaira Tokyo, 187-0011, Japan;(9) Present address: Laboratory of Clinical Plant Science, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo Tokyo, 113-8657, Japan |
| Abstract: | Fusarium head blight (FHB) is a devastating disease of small grain cereal crops caused by the necrotrophic pathogen Fusarium graminearum and Fusarium culmorum. These fungi produce the trichothecene mycotoxin deoxynivalenol (DON) and its derivatives, which enhance the disease development during their interactions with host plants. For the self-protection, the trichothecene producer Fusarium species have Tri101 encoding trichothecene 3-O-acetyltransferase. Although transgenic expression of Tri101 significantly reduced inhibitory action of DON on tobacco plants, there are several conflicting observations regarding the phytotoxicity of 3-acetyldeoxynivalenol (3-ADON) to cereal plants; 3-ADON was reported to be highly phytotoxic to wheat at low concentrations. To examine whether cereal plants show sufficient resistance to 3-ADON, we generated transgenic rice plants with stable expression and inheritance of Tri101. While root growth of wild-type rice plants was severely inhibited by DON in the medium, this fungal toxin was not phytotoxic to the transgenic lines that showed trichothecene 3-O-acetylation activity. This is the first report demonstrating the DON acetylase activity and DON-resistant phenotype of cereal plants expressing the fungal gene. S. Ohsato and T. Ochiai-Fukuda should be considered as joint first authors. |
| Keywords: | Deoxynivalenol (DON) Fusarium head blight Mycotoxin inactivation Protein synthesis inhibitor Trichothecene 3-O-acetyltransferase |
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