Construction of new stable strain over-expressing the glucose isomerase of the Streptomyces sp. SK strain |
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Authors: | Monia Mezghani Mohamed Ali Borgi Radhouane Kammoun Hedi Aouissaoui Samir Bejar |
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Institution: | Laboratoire d’Enzymes et de Métabolites des Procaryotes, Centre de Biotechnologie de Sfax, BP “K” 3038 Sfax, Tunisia |
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Abstract: | In order to over express the xylA gene of Streptomyces sp. SK strain, it was cloned under the control of the constitutive ermE-up promoter. This construct was integrated through site-specific recombination process into the chromosome of a Streptomyces violaceoniger glucose isomerase deficient strain using the non-replicative vector pTS55. The resulting CBS4 strain shows a perfect stability in the absence of selection pressure. Its glucose isomerase activity was about four and nine-fold greater, than that obtained from Streptomyces sp. SK, respectively fully induced or not by xylose. |
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Keywords: | Glucose isomerase Over-expression CBS4 strain ermE-up promoter |
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