| Institution: | 1. Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Pernambuco, Av. da Engenharia, s/n, Cidade Universitária, 50740-600 Recife-PE, Brazil;2. Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Pernambuco, Av. da Engenharia, s/n, Cidade Universitária, 50740-600 Recife-PE, Brazil
Laboratório de Farmacognosia, Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Rua Prof. Artur de Sá, s/n, Cidade Universitária, 50740-521 Recife-PE, Brazil;3. Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco, Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-420 Recife-PE, Brazil;4. Laboratório de Farmacognosia, Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Rua Prof. Artur de Sá, s/n, Cidade Universitária, 50740-521 Recife-PE, Brazil |
| Abstract: | The aim of this study was to evaluate the phytochemical composition, antioxidant, and antimicrobial potential of crude extract and fractions of Punica granatum leaves. The extract was produced by turbo extraction, after which hexanic, ethyl acetate, and aqueous fractions were obtained by partitioning. The chemical analyses were performed by thin layer chromatography and high-performance liquid chromatography, and the antioxidant activities were assayed by DPPH. and ABTS.+. Minimal inhibitory and bactericidal concentrations (MIC/MBC) were applied to twenty-two bacteria. Most strains susceptible to extract/fractions and resistant to antibiotics were selected, and ampicillin, azithromycin, ciprofloxacin, and gentamicin were associated with the ethyl acetate fraction (EAF) against multidrug-resistant strains in modulatory and checkboard models. The data from chromatographic analyses showed flavonoids and tannins in the extract, as well as the enrichment of EAF in phenols, mainly flavonoids. The flavonoids were connected to the electron transfer activity demonstrated in the DPPH. and ABTS.+ assays. Gram-positive strains are more susceptible to EAF. The subinhibitory concentrations of P. granatum enhanced the antimicrobial activity of the agents and reduced the EAF individual MIC, and the combination of EAF and antibiotics demonstrated a synergistic effect. These results present a promising approach for developing a therapy in which antioxidant extracts and fractions can be used in combination with antibiotics. |
