Evaluation of Antioxidant,Anti-Inflammatory and Antityrosinase Potential of Extracts from Different Aerial Parts of Rhanterium suaveolens from Tunisia |
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Authors: | Sabrine Chelly Meryam Chelly Cristina Occhiuto Francesco Cimino Mariateresa Cristani Antonina Saija Maria Sofia Molonia Giuseppe Ruberto Valeria D'Angelo Maria Paola Germanò Laura Siracusa Hanen Bouaziz-Ketata Antonio Speciale |
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Institution: | 1. Toxicology-, Microbiology- and Environmental Health Laboratory (RL 17ES06), Faculty of Sciences, University of Sfax, 3000 Sfax, Tunisia;2. Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Viale Annunziata, 98168 Messina, Italy;3. Istituto di Chimica Biomolecolare del Consiglio Nazionale delle Ricerche (ICB-CNR), Via Paolo Gaifami, 18, 95126 Catania, Italy;4. Istituto di Chimica Biomolecolare del Consiglio Nazionale delle Ricerche (ICB-CNR), Via Paolo Gaifami, 18, 95126 Catania, Italy
These authors share senior authorship.;5. Toxicology-, Microbiology- and Environmental Health Laboratory (RL 17ES06), Faculty of Sciences, University of Sfax, 3000 Sfax, Tunisia
These authors share senior authorship.;6. Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Viale Annunziata, 98168 Messina, Italy
These authors share senior authorship. |
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Abstract: | The genus Rhanterium (Asteraceae) is a widely distributed medicinal plant throughout western North Africa and some Rhanterium species are used in folk medicine. The aim of research was to investigate methanolic extracts from different parts (flowers, leaves, and stems) of Tunisian Rhanterium suaveolens as potential sources of bioactive products useful for healthy purposes. In particular, were analyzed the phenolic composition of these extracts and their antioxidant, anti-inflammatory, and anti-tyrosinase properties. The phytochemical analyses were performed using standard colorimetric procedures, HPLC-DAD and HPLC-DAD-ESI-MS. Then, several in vitro cell-free assays have been used to estimate the antioxidant/free radical scavenging capability of the extracts. Moreover, in vitro, and in vivo anti-melanogenesis activities of these extracts were tested, respectively, with the tyrosinase inhibition assay and the Zebrafish embryo model. Finally, the anti-inflammatory potential of these extracts in an in vitro model of acute intestinal inflammation in differentiated Caco-2 cells was evaluated. The R. suaveolens extracts under study appeared particularly rich in flavonols and hydroxycinnamic acids and all extracts appeared endowed with good antioxidant/free radical scavenging properties, being the flower extracts slightly more active than the others. Moreover, R. suaveolens flowers extract was able to inhibit in vitro tyrosinase activity and exhibited bleaching effects on the pigmentation of zebrafish embryos. Furthermore, all extracts showed good anti-inflammatory activity in intestinal epithelial cells as demonstrated by the inhibition of TNF-α-induced gene expression of IL-6 and IL-8. R. suaveolens aerial parts may be considered as a potential source of whitening agents, as well as of agents for the treatment of disorders related to oxidative stress and inflammation. |
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Keywords: | Rhanterium suaveolens anti-inflammatory activity HPLC/ESI-MS tyrosinase activity interleukins |
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