| 肝细胞靶向性系统表达载体的构建 |
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| 引用本文： | 王传玺,韩金祥,鲁艳芹,高雪芹,张翠.肝细胞靶向性系统表达载体的构建[J].生物技术通讯,2004,15(5):429-432. |
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| 作者姓名： | 王传玺 韩金祥 鲁艳芹 高雪芹 张翠 |
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| 作者单位： | 山东省医药生物技术研究中心,卫生部生物技术药物重点实验室,济南,250062 |
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| 基金项目： | 国家自然科学基金(30371328)，山东省自然科学基金(Q99015) |
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| 摘 要： | 在肝脏疾病的基因治疗过程中，为把目的基因定向导入靶细胞，构建了具有靶向性的逆转录病毒的包装细胞．即应用RT-PCR方法分别反转录并扩增env,pres2基因，将它们分别克隆至pGEM-T载体上，经测序正确后，将一目的基因亚克隆在pcDNA3．1(-)表达载体上，然后将另一目的片段从T载体上切下，克隆至经同样酶切的重组表达载体中。从而成功地构建了肝细胞靶向性系统的表达载体pcDNA3．1(-)-env-pres2和pcDNA3．1(-)-pres2-env。
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| 关 键 词： | 靶向性 肝细胞 pcDNA3 表达载体 pres2基因 包装细胞 逆转录病毒 T载体 目的基因 克隆 |
| 文章编号： | 1009-0002(2004)05-0429-04 |
| 修稿时间： | 2004年4月12日 |
Construction of the expression vector of PHSA-R with Env protein |
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| WANG Chuan-xi,HAN Jin-xiang,LU Yan-qin,GAO Xue-qin,ZHANG CuiKey Bio-pharmacy Lab of Health Ministry,Shandong Medicinal Biotechnology Center,Jinan ,China.Construction of the expression vector of PHSA-R with Env protein[J].Letters in Biotechnology,2004,15(5):429-432. |
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| Authors: | WANG Chuan-xi HAN Jin-xiang LU Yan-qin GAO Xue-qin ZHANG CuiKey Bio-pharmacy Lab of Health Ministry Shandong Medicinal Biotechnology Center Jinan China |
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| Institution: | WANG Chuan-xi,HAN Jin-xiang,LU Yan-qin,GAO Xue-qin,ZHANG CuiKey Bio-pharmacy Lab of Health Ministry,Shandong Medicinal Biotechnology Center,Jinan 250062,China |
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| Abstract: | The env?pres2 gene coding Env protein and polymerized human serum albumin receptor(PHSA-R) were amplified by RT-PCR, they were cloned respectively into vector pGEM-T. After DNA sequences were determined, one insert gene was subcloned into expression vector pcDNA3.(-), then the other insert gene which was digested by two restriction endonucleases was subcloned into recombined plasmid with the same sites, the fusion expression vectors were named pcDNA3.1(-)-env-pres2 and pcDNA3.1(-)-pres2-env. |
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| Keywords: | hepatitis B virus ribozyme Env protein polymerized human serum albumin receptor |
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