Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA) |
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| Authors: | Cheng-Yu Hung Kuo-Hsiung Wang Chi-Chun Huang Xun Gong Xue-Jun Ge Tzen-Yuh Chiang |
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| Institution: | (1) Department of Life Sciences, Cheng-Kung University, 1, University Road, Tainan, 701, Taiwan;(2) Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, P.R. China;(3) State Key Laboratory of Biocontrol School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, P.R. China |
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| Abstract: | We report 11 novel microsatellite primer pairs for the wild tea, Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura. These simple sequence repeat markers were tested in 24 samples collected from wild tea populations, and in cultivars and
C. japonica. The number of alleles ranged from 4 to18. The expected (H
E) and observed (H
O) heterozygosity were 0.687–0.946 and 0.042–0.792, respectively. All loci were significantly deviated from Hardy-Weinberg
expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the rare species.
Significant LD was discovered in most loci. These primers may provide a tool for understanding demography and population structure
in wild tea. |
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| Keywords: | Camellia sinensis Heterozygosity Microsatellite fingerprinting PIMA RAPD-PCR enrichment |
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