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Redox activities in plasma membrane vesicles isolated from papaya (Carica papaya) leaves
Authors:Silvia Paredes  Ignacio Núñez de Cstro  Miguel A. Medina
Affiliation:(1) Laboratorio de Bioquímica, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, Spain
Abstract:Plasma membrane vesicles were isolated from papaya (Carica papaya) leaf cells by a two-phase partition system. Redox activities of these vesicles were determined by ferricyanide reduction and NADH oxidation. Ferricyanide reductase activity was accompanied by continuous acidification of the medium and was stimulated by fusicoccin. NADH oxidase activity was inhibited by catalase.Abbreviations FC fusicoccin - PCMBS parachloromercuri-benzenesulfonic acid
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