Toxicity of prostaglandins A1 and A2 for cells in culture

Summary Prostaglandins A₁ and A₂, in concentrations near 3×10⁻⁵ M, produced striking toxicity to muscle, skin and liver cells in culture. Prostaglandins E and F_2α were much less active in this regard. Toxicity could be measured by reduction in viable cell number, protein and DNA synthesis in the cultures. The sensitivity of cultured cells was related to their age and population density. Dense cultures were sensitive early, in the first 2 days, and resistant after they manifested confluent growth. Sparse cultures remained sensitive later while they continued DNA synthesis and active cell division. It is hypothesized that the prostaglandin A effect is related to active cell division and DNA synthesis. Supported by USPHS NIAMDD 1RO1 AM 14650 and the Muscular Dystrophy Associations of America, Inc.