Phylogenetic characterization of ineffective Frankia in Alnus glutinosa (L.) Gaertn. nodules from wetland soil inoculants |
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| Authors: | D J Wolters C Van Dijk E G Zoetendal & A D L Akkermans |
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| Institution: | Department of Plant Microorganism Interactions, Centre for Terrestrial Ecology, Netherlands Institute of Ecology, PO Box 40, 6666 ZG Heteren, the Netherlands,;Department of Microbiology, Wageningen Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, the Netherlands |
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| Abstract: | Ineffective Frankia endophytes were retrieved from various wet soils by using Alnus glutinosa clones as trapping plants. No pure cultures could be isolated from these ineffective nodules. Therefore, the phylogenetic position of these endophytes was determined by sequence analysis of cloned PCR products of bacterial 16S rDNA, derived from nodules. The results showed that all nodule endophytes belong to a hitherto undescribed cluster of the Frankia phylogenetic tree. The position of these uncultured ineffective Frankia nodule endophytes is different from that of the ineffective Frankia isolates derived from A. glutinosa nodules, even when originating from the same geographical location. This suggests a bias in current isolation techniques. |
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| Keywords: | ineffective Frankia Alnus 16S rDNA phylogeny |
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