Cold-induced mRNAs accumulate with different kinetics in barley coleoptiles |
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Authors: | L Cattivelli D Bartels |
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Institution: | (1) Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30, Germany;(2) Present address: Experimental Institute for Cereal Research, Via S. Protaso, 308, I-29017 Fiorenzuola d'Arda (PC), Italy |
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Abstract: | The effect of cold treatment on gene expression in two different barley (Hordeum vulgare L.) cultivars has been studied. Cold stress induced a set of new mRNAs as determined by in-vitro translation of coleoptile RNA obtained from control and stressed seedlings. These mRNAs accumulated with different kinetics, and the cold-induced proteins could be grouped into five categories. The first category (a) is represented by a single protein with Mr of 75 kDa that reaches its highest level of expression after 6 h at 5°C. This polypeptide readily accumulates in the plant tissues and it can be detected when proteins separated by two-dimensional electrophoresis are stained with silver nitrate. The other polypeptides appear later during the 1- to 4-d stress period (protein groups b and c), increase (group d), or decrease during the period of treatment (group e). Only minor differences between the two cultivars with different cold-resistance capacities were found when the in-vitro translation products were compared. The results obtained demonstrate that several mRNAs are specifically expressed as a response to cold treatment in barley coleoptiles.Abbreviations 2-D
two-dimensional
- IEF
isoelectrofocusing
- Mr
relative molecular weight
- poly(A)
polyadenylated
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis |
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Keywords: | Cold acclimation Hordeum (cold induced mRNA) mRNA expression Stress proteins |
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