| 小鼠神经系统内强啡肽B免疫活性物质及其高压液相层析(HPLC)分析 |
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| 引用本文： | 祝元祥,王成海,崔瑞耀.小鼠神经系统内强啡肽B免疫活性物质及其高压液相层析(HPLC)分析[J].生理学报,1986(2). |
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| 作者姓名： | 祝元祥 王成海 崔瑞耀 |
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| 作者单位： | 第二军医大学生理学教研室 上海
(祝元祥，王成海)，青岛医学院 进修教师(崔瑞耀) |
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| 摘 要： | 强啡肽B是一种新发现的阿片肽。本工作以自制的兔抗强啡肽 B血清建立灵敏的放射免疫测定法,测定了小鼠神经系统与垂体内强啡肽 B免疫活性物质的含量,其中以垂体、下丘脑含量最高。 放射免疫测定结合 Sephadex C-50 层析和HPLC分析的结果表明,小鼠脑内强啡肽B免疫活性物质的主要成分是强啡肽 B,另外也包括了一定量的强啡肽-32和另一未知的分子量更大的成分,但不像大鼠还含有强啡肽 B-29。这种种属特异性的意义有待进一步研究。
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| 关 键 词： | 强啡阶B 强啡肽—32 强啡肽B—29 放射免疫测定 高压液相层析(HPLC) 垂体 |
IMMUNOREACTIVE DYNORPHIN B IN CENTRAL NERVOUS SYSTEM OF MOUSE AND ITS HIGH PERFORMANCE LIQUID CHROMATOGRAPH (HPLC) ANALYSIS |
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| ZHU YUAN-XIANG,WANG CHENG-HAI,CUI RUI-YAO.IMMUNOREACTIVE DYNORPHIN B IN CENTRAL NERVOUS SYSTEM OF MOUSE AND ITS HIGH PERFORMANCE LIQUID CHROMATOGRAPH (HPLC) ANALYSIS[J].Acta Physiologica Sinica,1986(2). |
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| Authors: | ZHU YUAN-XIANG WANG CHENG-HAI CUI RUI-YAO |
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| Institution: | ZHU YUAN-XIANG,WANG CHENG-HAI,CUI RUI-YAO Department of Physiology,Second Military Medical College Shanghai |
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| Abstract: | Dynorphin B is a novel opioid tridecapeptide. We have developed a sensitiveradioimmunoassay for dynorphin B and its related peptides (dynorphin-32 anddynorphin B-29) and determined the immunoreactive dynorphin B levels in pitui-tary and some other areas of mouse central nervous system. The highest concentra-tions were found in pituitary and hypothalamus. Sephadex G-50 chromatography and HPLC reveal that immunoreactivedynorphin B in mouse brain mainly is dynorphin B, and also comprises certainamount of dynorphin-32 and an unknown component with higher molecular weight. |
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| Keywords: | dynorphin B dynorphin-32 dynorphin B-29 radioimmunoassay high performace liquid chromatograph pituitary |
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