Host-vector systems for the thermotolerant methanol-utilizing bacteriaMethylophilus spp. KISRI-Strains |
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| Authors: | Dr Nuzha M Al-Taho Sami H Al-Amad Fadilah A Al-Salameen Ekkehard Spanier Mohammad Z Haider |
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| Institution: | (1) Biotechnology Department, Kuwait Institute for Scientific Research, P.O. Box 24885, 13109 Safat, Kuwait;(2) Present address: Akademie der Wissenschaften, der DDR, Institut fur Biotechnologie, Permoserstraße 15, 7050 Leipzig, Germany |
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| Abstract: | Fourteen recombinant plasmids were constructed by inserting fragments of pSAS, a naturally occurring plasmid ofMethylophilus spp. KISRI-5, into the multiple cloning sites of pUC19. Six recombinants and three knownEscherichia coli plasmids were used to transform three thermotolerant methylotrophic KISRI strains by use of an optimized protocol of electroporation. Analysis of transformants for plasmid DNA showed that all plasmids were stable in the methylotrophic hosts. These studies offer opportunities to developMethylophilus spp. as host-vector systems. |
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