猴免疫缺陷病毒特异性免疫复合物沉着与猴AIDS多系统病变关系的探讨

目的观察猴免疫缺陷病毒(SIV)感染后,病毒特异性免疫复合物(IC)在不同时间、不同组织的沉着情况,初步研究其与AIDS多系统病变的联系。方法对8只不同时间感染SIV的恒河猴及1只未感染SIV的猴进行尸检以获取多系统组织标本,进行连续切片和IgG、C3、SIV p27免疫荧光染色,并对结果进行比较分析。结果IgG、C3、p27在多个猴、多种组织的相同位置出现相同模式的荧光表达,证明存在IC的沉着;其中脑血管周(8/8),心肌间微血管(6/8)、和淋巴结副皮质(6/8)及生发中心(5/8)是阳性率最高的部位,肾小球及肾间质、肠黏膜固有层也有较多IC的沉着,且在感染中、晚期IC出现的比例更高。结论SIV感染后出现广泛的SIV-IC沉积,且随病情的进展而加重;IC可能是SIV导致AIDS多系统病变的主要形式。针对此过程进行研究,可帮助了解AIDS并发多系统器官病变的机制及研究新的治疗方法。

Relation of Deposition of Simian Immunodeficiency Virus-Specific Immune Complex to SAIDS Multisystemic Pathologic Changes

Objective To observe the deposition of SIV specific immune complex in different organs at different time-points after SIV infection,and explore its possible relationship with multisystemic pathological changes in simian acquired immunodeficiency syndrome(SAIDS).Methods\ 8 SIV-infected rhesus monkeys and 1 uninfected monkey were autopsied and sampled.Serial sections were prepared,immune fluorescence staining of IgG,C3 and SIV p27 were performed,and the results were compared and analyzed.Results\ IgG,C3 and SIV p27 fluorescence expressed similarly at the same site in most monkeys,indicating the presence of SIV-specific immune complex.Among those positions,cerebral perivascular space(8/8),myocardial interstitial vessel(6/8),paracortex(5/8) and germinal center(5/8) of lymph nodes were the main sites with a higher positive rate.Their positive rates were elevated during the late phase after infection.Conclusion After SIV infection,viral specific immune complex is deposited widely,and increased remarkably with disease progression.SIV may lead to AIDS associated multisystem complication through IC deposition.Further research on it may be helpful to understand how SIV infection induces multisystem pathological changes,and it is also a promising target for developing new treatment strategy.