Specific molecular marker of the genes controlling linolenic acid content in rapeseed |
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Authors: | C Jourdren P Barret D Brunel R Delourme M Renard |
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Institution: | (1) INRA, Station d'Amélioration de Plantes, BP 29, F-35650 Le Rheu, France;(2) INRA, Station d'Amélioration de Plantes, Route de Saint Cyr, F-78026 Versailles Cedex, France |
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Abstract: | In rapeseed, which is an agronomically important oilseed, variation in the linolenic acid content of the oil has been obtained through chemical mutagenesis treatment. Conventional breeding of this quantitative trait, however requires specific molecular markers. By means of biochemical experiments, we have established that the induced variation in linolenic acid content is associated with the fad3 gene encoding the microsomal 15 desaturase. Using a pair of primers specific to this gene and a doubled haploid progeny derived from a low linolenic x high linolenic acid F1hybrid, we have identified a polymorphism of the fad3 alleles between the low- and the high-linolenic acid genotypes. The structure exon/intron of the fad3 DNA sequence seems to be very similar to that of the Arabidopsis fad3 gene. The choice of the primer pair allows specific amplification of one of the two rapeseed fad3 genes. The value and contribution of specific markers to conventional plant breeding is discussed. |
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Keywords: | fad3 gene 15 Microsomal desaturase" target="_blank">gif" alt="Delta" align="BASELINE" BORDER="0">15 Microsomal desaturase Linolenic acid Mutant Rapeseed Specific gene mapping |
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