慢性溴氰菊酯摄取对大鼠胃黏膜损伤及P物质的表达的影响

目的:探索溴氰菊酯慢性中毒对大鼠胃黏膜的病理损伤及损伤后P物质表达的影响.方法:实验组大鼠分别给予不同荆量溴氰菊酯灌胃,对照组仅给予橄榄油.取胃体部组织,制成石蜡切片,HE及免疫组织化学染色,镜下观察胃黏膜损伤及胃黏膜组织P物质的表达情况,并对损伤程度进行累计积分、壁细胞计数.结果:高剂量组大鼠胃粘膜慢性损害病理积分与各组相比差异均有显著性(P＜0.05);壁细胞计数与对照组相比较其差异有P＜0.05).免疫组化发现SP在实验组大鼠胃黏膜组织中呈强阳性表达,在对照组则呈弱阳性表达;平均光密度(OD)值显示高、中剂量组之间及与其余各组之间差异均有显著(P＜0.05l.结论:溴氰菊酯对大鼠胃黏膜有较明显的慢性损伤,SP可能参与胃黏膜局部病变.

Chronic Effects of Deltamethrin on the Gastric Mucosa Injury and the Expression of Substance P in Rats

Objective:To investigate the chronic toxicity of deltamethrin on rats'gastric mueosa injury and to investigate the expression of substance P in the gastric mucosa.Methods:Wistar rats were given different doses of deltamethrin dissolved in the olive oil via esophagus,and the control group received olive oil only.Paraffin sections were made from the fundus of stomach.After HE and immunohistochemical staining,the degrees of gastric mucosa injury and the expression of substance P were detected.Results:Compared with the rats in other groups,the degrees of gastric mucosa damage in high-dose group were higher (P ＜0.05),the number of parietal cells in high-dose group was higher too (P＜0.05).Expression of substance P was found in cells of the rat gastric mueosa in all groups and there was weak expression in the control group.The average optical density (OD) values found in the high and middle dose groups were higher than those of another groups (P＜0.05).Conclusion:Deltamethrin has apparently chronic harmful effect on rats'gastric mucosa injury and SP may be involved in the pathogenesis of the localized gastric lesions.