Axillary bud proliferation and organogenesis of <Emphasis Type="Italic">Euphorbia pulchurrima</Emphasis> winter rose |
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Authors: | K?A?Pickens Email author" target="_blank">Z?M?ChengEmail author R?N?Trigiano |
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Institution: | (1) Department of Plant Sciences, University of Tennessee, 37996-4500 Knoxville, TN;(2) Department of Entomology and Plant Pathology, University of Tennessee, 37996-4560 Knoxville, TN |
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Abstract: | Summary Protocols for both axillary bud proliferation and shoot organogenesis of Euphorbia pulchurrima Winter RoseTM were developed using terminal buds and leaf tissues. Greenhouse-grown terminal buds were placed on Murashige-Skoog (MS) basal
medium supplemented with various concentrations of either benzlyaminopurine (BA) or thidiazuron (TDZ). Explants produced the
greatest number of axillary buds on media containing between 2.2 and 8.8 μM BA. The number of explants that produced axillary buds increased with increasing BA concentration. TDZ at concentrations
between 2.3 and 23.0 μM caused hyperhydricity of shoots and were not effective in promoting shoot proliferation. The most calluses and shoots were
produced from leaf midvein sections from in vitro grown plants placed on the medium containing 8.8–13.3 μM BA and 17.1 μM indole-3-acetic acid (IAA) for 1 mo. before transferring to the medium containing only BA. Adventitious buds were produced
only from red-pigmented callus, and explants that produced callus continued to produce adventitious shoots in the presence
of IAA. Five-mo.-old shoots derived from shoot culture or organogenesis rooted readily in artificial soil with or without
treatment with indolebutyric acid, and were acclimatized in the greenhouse. |
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Keywords: | poinsettia terminal buds regeneration organogenesis leaf sections |
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