IgG production kinetics in serum free media |
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Authors: | John P Tharakan Pao C Chau |
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Institution: | (1) Department of Ames (Chemical Engineering), University of California, San Diego, 92093 La Jolla, CA |
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Abstract: | Summary A murine hybridoma (455) was cultured in four different serum free media formulations, and a newborn calf serum supplemented medium was used as a basis of comparison. The serum supplemented medium supports a higher cell growth rate and results in a higher IgG titer. However, the antibody secretion rate on a per cell basis is higher in the serum free media, indicating that serum could be inhibitory to antibody secretion. The results identify the possibility of a least eliminating serum during the monoclonal antibody production phase. |
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