| 用“增强子陷阱”技术构造并筛选果蝇UAS/GAL4系统中GAL4新品系及脑基因表达图谱数据库的开发 |
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| 引用本文： | 刘晓隽,袁小京,孙侃,帅袆春,宋庆轩,王磊,邵丽莎,赵昕宇,吕义晟,吕玉兵,王连章,钟毅.用“增强子陷阱”技术构造并筛选果蝇UAS/GAL4系统中GAL4新品系及脑基因表达图谱数据库的开发[J].生物化学与生物物理进展,2008,35(6):645-649. |
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| 作者姓名： | 刘晓隽 袁小京 孙侃 帅袆春 宋庆轩 王磊 邵丽莎 赵昕宇 吕义晟 吕玉兵 王连章 钟毅 |
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| 作者单位： | 清华大学生物科学与技术系,北京,100084 |
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| 摘 要： | "增强子陷阱"技术是建立果蝇脑全基因组表达图谱及其数据库的重要方法.筛选获得新特异表达的GAL4品系,可为进一步研究果蝇脑神经在学习记忆功能提供强有力的基因工具.通过"增强子陷阱"技术来获得果蝇突变体,并与报告转基因果蝇(UAS-EGFP)杂交,用荧光显微镜观察成年果蝇脑内荧光分布,从而获得该突变体的脑基因表达图谱,在此基础上利用JavaScript来建立果蝇脑全基因组表达数据库.目前获得基因突变体果蝇2 677种,大部分在果蝇脑中有表达,其中在果蝇嗅觉学习记忆相关脑区蘑菇体表达的基因有368个,且有部分基因特异地表达在某些传导通路上.这些果蝇基因突变体库及其表达图谱为进一步研究各基因的功能及作为遗传工具来研究各脑区结构和功能提供极大方便.
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| 关 键 词： | 增强子陷阱 突变体文库 报告转基因果蝇 蘑菇体 表达图谱 增强子 技术构造 筛选 果蝇 系统 新品系 基因表达图谱 数据库 开发 Database Expression Pattern Establishment Method Enhancer Trap In vivo Drosophila Adult 大方 结构和功能 遗传 |
| 收稿时间： | 2008/4/18 0:00:00 |
| 修稿时间： | 2008/5/12 0:00:00 |
Genomic Screen for Expression Pattern in Adult Drosophila Brains In vivo by Enhancer Trap Method and Establishment of Expression Database |
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| LIU Xiao-Jun,YUAN Xiao-Jing,SUN Kan,SHUAI Yi-Chun,SONG Qing-Xuan,WANG Lei,SHAO Li-Sh,ZHAO Xin-Yu,LU Yi-Sheng,LU Yu-Bing,WANG Lian-Zhang and ZHONG Yi.Genomic Screen for Expression Pattern in Adult Drosophila Brains In vivo by Enhancer Trap Method and Establishment of Expression Database[J].Progress In Biochemistry and Biophysics,2008,35(6):645-649. |
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| Authors: | LIU Xiao-Jun YUAN Xiao-Jing SUN Kan SHUAI Yi-Chun SONG Qing-Xuan WANG Lei SHAO Li-Sh ZHAO Xin-Yu LU Yi-Sheng LU Yu-Bing WANG Lian-Zhang and ZHONG Yi |
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| Institution: | Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China |
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| Abstract: | |
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| Keywords: | enhancer trap method mutant libraries reporter transgenic flies mushroom body expression pattern |
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