Cytochemical localization of glycoconjugates in rat peritoneal mast cells during degranulation |
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| Authors: | Kam Choi Poon P L Sannes J A V Simson and S S Spicer |
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| Institution: | (1) Departments of Anatomy and Pathology, School of Medicine, Medical University of South Carolina, 29403 Charleston, South Carolina, USA;(2) Present address: Department of Health Sciences, The Johns Hopkins University, School of Hygiene and Public Health, 615 N. Wolfe Street, 21205 Baltimore, Maryland, USA |
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| Abstract: | Summary Cytochemical methods for the localization of glycoconjugates including concanavalin A-horseradish peroxidase (ConA-HRP) and dialysed iron were used to study the distribution of glycoconjugates in mast cell granules during degranulation. The ConA-HRP method revealed intense staining of discharged mast cell granules. Dialysed iron staining was seen at the granule periphery, with extruded granules exhibiting more intense staining than undischarged granules.Some of the work reported herein was performed in partial fulfillment of the requirements for a Ph.D. degree. |
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