百合花粉母细胞间细胞融合期间腺苷三磷酸酶活性的细胞化学定位及其与染色质胞间转移的关系

用标准的磷酸铅沉淀的细胞化学方法,对百合花粉母细胞间染色质穿壁运动期间及其前后三个时期中的腺苷三磷酸酶(ATP 酶)活性进行了超微结构的定位。结果表明:(1)在穿壁前,ATP 酶活性主要定位于质膜、胞间连丝及细胞间隙;在内质网、高尔基体、质体和某些局部的基质(groundplasm)中,也表现有 ATP 酶活性反应的产物;但在染色质和核仁中,一般都没有这种反应。(2)在穿壁时,染色质从一个细胞穿壁转移到另一个相邻细胞,同时看到染色质和核仁内出现密集的 ATP 酶活性反应产物;在内质网和高尔基体的腔内以及质体的片层上也产生明显的 ATP 酶活性反应;而在质膜、胞间连丝及细胞间隙内 ATP 酶活性明显降低,甚至看不到明显的活性反应。(3)在穿壁后,质膜及细胞间隙中又产生明显的 ATP 酶活性反应产物,但核内染色质上的 ATP 酶活性则显著降低,而核仁内则仍有较高的活性。同前二个时期一样,内质网、高尔基体和质体上的 ATP 酶仍表现明显的活性反应。最后讨论了三个不同发育时期 ATP 酶活性及其分布部位的改变与染色质胞间转移的关系。

CYTOCHEMICAL LOCALIZATION OF ADENOSINE TRIPHOSPHATASE ACTIVITY DURING CYTOMIXIS IN POLLEN MOTHER CELLS OF DAVID LILY AND ITS RELATION TO THE INTERCELLULAR MIGRATING CHROMATIN SUBSTANCE

Standard lead precipitation procedures have been used to examine the localization of ATPase activity during cytomixis in pollen mother cells of Lilium davidii var.willmottiae (Wilson) Roffill.Before cytomixis,cells at this stage of development show ATPase activity on plasma membrane,in the endoplasmic reticulum,dictyosomes, plastids,plasmodesmata,and in part of the groundplasm;however,there is no ATPase activity on the chromatin and nucleolus.During cytomixis,the chromatin substance begin to transfer from one cell to an adjacent cell,reaction product indicating ATPase activity is observed associated with the chromatin and nucleolus.ATPase activity is also found with the cistenae of both endoplasmic reticulum and dictyosomes,and some plastids.There is no deposition of ATPase reaction product associated with the plasm membrane and intercellular spaces.After cytomixis,the chromatin is little or no depo- sition of enzyme reaction product.ATPase activity,however,is consistenlly found within the intercellular space and on the plasm membrane,and also occur in the endo- plasmic reticulum,dictyosome and plastid. The presence or absence of ATPase activity in the cell structure of pollen mother cells before,during or after cytomixis is discussed in relation to the active uptake or export of water for short-distance transport.It is also suggested that the intensive ATPase activity in the nucleus during cytomixis of pollen mother cells is evidence for a transport system involved in the active movement of the intercellular migrating chro- matin substance.