Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis |
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| Authors: | Štefan Čikoš Alexandra Bukovská Juraj Koppel |
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| Affiliation: | 1.Institute of Animal Physiology, Slovak Academy of Sciences,Ko?ice,Slovakia |
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| Abstract: | Background Fluorescent data obtained from real-time PCR must be processed by some method of data analysis to obtain the relative quantity
of target mRNA. The method chosen for data analysis can strongly influence results of the quantification. |
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