| 丹参病程相关蛋白基因（SmPR-10）的生物信息学及表达模式分析 |
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| 引用本文： | 生华,刘梅,化文平,王喆之.丹参病程相关蛋白基因（SmPR-10）的生物信息学及表达模式分析[J].武汉植物学研究,2011,29(3):340-346. |
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| 作者姓名： | 生华 刘梅 化文平 王喆之 |
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| 作者单位： | 教育部药用植物资源与天然药物化学重点实验室,西北濒危药材资源开发国家工程实验室,陕西师范大学生命科学学院,西安,710062 |
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| 基金项目： | 国家自然科学基金资助项目(31000134); 陕西省科技研究发展(攻关)计划资助项目(2006K16-G15) |
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| 摘 要： | 对丹参EST序列进行Blast分析,获得了一条病程相关蛋白10（Pathogenesis-related protein10,PR-10）基因,命名为：SmPR-10（GenBank注册号：HM439764）。分别从cDNA和gDNA水平克隆得到该基因,其序列无内含子,包含一个长为486bp的完整开放读码框,编码161个氨基酸残基。生物信息学分析显示,SmPR-10所编码的蛋白SmPR-10具有Betv1结构域,属于病程相关蛋白10家族,其相对分子量为17·47kD,为稳定的酸蛋白;无信号肽、膜锚定或跨膜结构域,为亲水性蛋白。实时定量PCR结果分析表明,SmPR-10基因主要在丹参茎中表达,其表达受到病原菌和茉莉酸甲酯逆境信号的诱导,显示SmPR-10基因可能在植物防御反应中发挥作用。
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| 关 键 词： | SmPR-10 生物信息学分析 表达模式 |
Bioinformatics and Expression Pathogenesis-related Protein 10 Gene(SmPR-10) from Salvia miltiorrhiza Bunge |
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| SHENG Hua,LIU Mei,HUA Wen-Ping,WANG Zhe-Zhi.Bioinformatics and Expression Pathogenesis-related Protein 10 Gene(SmPR-10) from Salvia miltiorrhiza Bunge[J].Journal of Wuhan Botanical Research,2011,29(3):340-346. |
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| Authors: | SHENG Hua LIU Mei HUA Wen-Ping WANG Zhe-Zhi |
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| Institution: | SHENG Hua,LIU Mei,HUA Wen-Ping,WANG Zhe-Zhi*(Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry,National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China,College of Life Sciences,Shaanxi Normal University,Xi'an 710062,China) |
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| Abstract: | After analysis of Salvia miltiorrhiza EST sequences,one sequence was found had high homology with pathogenesis-related protein 10.The cDNA and DNA sequence of the gene were cloned and named as SmPR-10(GenBank accession number:HM439764).SmPR-10 without intron contained a single opening reading frame of 486 bp encoding 161 amino acid-peptides.SmPR-10 had a Bet v1 domain and was classed into the pathogenesis-related protein 10 family according to its sequence.Bioinformatics showed that the putative protein was... |
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| Keywords: | SmPR-10 Bioinformatics analysis Expression analysis |
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