人早孕子宫蜕膜催乳素分泌的调节

子宫内膜蜕膜化对胚泡植入与妊娠维持是非常重要的。为探讨蜕膜化维持的调节机制 ,本文研究了妊娠早期人子宫蜕膜细胞催乳素 (PRL)分泌的调节。结果表明 :(1)孕酮显著地刺激PRL的分泌。 (2 )雌激素的作用与其浓度有关 ,生理浓度的雌激素对PRL分泌无明显影响 ,而高水平的雌激素抑制孕酮的刺激作用。合适的雌孕激素比例对蜕膜化的维持是必要的。 (3)RU486明显地抑制PRL的分泌 ,故认为孕酮的作用至少是部分通过受体介导的机制。 (4 )高浓度的cAMP (≥ 10 -5mol/L)显著增加PRL的分泌 ,cAMP信号系统可能在蜕膜化反应中发挥重要作用。

Modulation of prolactin (PRL) secretion in human uterine decidual cells at early pregnancy

Decidualization is essential for implantation of embryo and maintenance of pregnancy in human. The mechanism of decidualization was investigated in this study by regulation of prolactin (PRL) release in cultured human decidual cells during first trimester of gestation. Progesterone significantly stimulated PRL secretion, but the effect of estrogen depended on its concentration, which was ineffective at the physiological level (10 -9 mol/L) but suppressed the stimulatory effect of progesterone at higher levels. Thus adequate proportion of estrogen and progesterone is important for the decidualization. Furthermore, RU486 dramatically inhibited PRL release, suggesting that the effect of progesterone was mediated, at least in part, through its receptor. cAMP at concentration higher than 10 -5 mol/L significantly increased PRL secretion, suggesting that the cAMP signal pathway might be involved in decidualization.