A new instrument for the rapid preparation of tissue slices |
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Authors: | C L Krumdieck J E dos Santos K J Ho |
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Affiliation: | Division of Laboratories and Research, New York State Department of Health, Albany, New York 12201 USA |
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Abstract: | The three methods most commonly used for removing RNase from preparations of DNase were compared under similar assay conditions for residual RNase activity. In order of their effectiveness they ranked: (1) bentonite, (2) agarose-UMP, (3) iodoacetate. Even the best of the single treatments did not eliminate all of the RNase, and it was discovered that a significant improvement was obtained when DNase was first passed over agarose-UMP and then treated with bentonite. |
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