Isoenzyme Polymorphism in Flowering Plants III Gel Electrophoretic Methods and Applications |
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Authors: | James L.,Brewbaker ,Mahesh D.,Upadhya ,Yrjö ,Mä kinen Timothy,Macdonald |
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Affiliation: | Department of Horticulture, University of Hawaii Honolulu, Hawaii 96822 |
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Abstract: | Gel electrophoretic methods and equipment which were devised or modified for rapid and economic application to angiosperm tissues are described. Staining schedules are outlined that proved effective on a wide variety of tissues for elucidation of isoenzymes with the following activities; eslerase, peroxidase, catalase, leucine amino peptidase, phosphatase, amylase, and glutamic-oxalacetic-transaminase. Illustrative applications are discussed. |
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