A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis |
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| Authors: | Erin Bakker Ute Achenbach Jeroen Bakker Joke van Vliet Johan Peleman Bart Segers Stefan van der Heijden Piet van der Linde Robert Graveland Ronald Hutten Herman van Eck Eric Coppoolse Edwin van der Vossen Jaap Bakker Aska Goverse |
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| Institution: | (1) Laboratory of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands;(2) Laboratory of Plant Breeding, The Graduate School of Experimental Plant Sciences, Wageningen University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands;(3) Keygene NV, P.O. Box 216, 6700 AE Wageningen, The Netherlands;(4) Cebeco Seeds, P.O. Box 139, 8200 AC Lelystad, The Netherlands;(5) HZPC R and D, P.O. Box 2, 9123 ZR Metslawier, The Netherlands;(6) Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands |
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| Abstract: | The resistance gene H1
confers resistance to the potato cyst nematode
Globodera rostochiensis and is located at the distal end of the long arm of chromosome V of potato. For marker enrichment of the H1 locus, a bulked segregant analysis (BSA) was carried out using 704 AFLP primer combinations. A second source of markers tightly linked to H1 is the ultra-high-density (UHD) genetic map of the potato cross SH × RH. This map has been produced with 387 AFLP primer combinations and consists of 10,365 AFLP markers in 1,118 bins (). Comparing these two methods revealed that BSA resulted in one marker/cM and the UHD map in four markers/cM in the H1 interval. Subsequently, a high-resolution genetic map of the
H1 locus has been developed using a segregating F1 SH × RH population consisting of 1,209 genotypes. Two PCR-based markers were designed at either side of the
H1 gene to screen the 1,209 genotypes for recombination events. In the high-resolution genetic map, two of the four co-segregating AFLP markers could be separated from the H1 gene. Marker EM1 is located at a distance of 0.2 cM, and marker EM14 is located at a distance of 0.8 cM. The other two co-segregating markers CM1 (in coupling) and EM15 (in repulsion) could not be separated from the
H1 gene.Communicated by J.G. Wenzel |
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