Genetic variability and population structure of Bergenia ciliata (Saxifragaceae) in the Western Himalaya inferred from DAMD and ISSR markers |
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| Institution: | 1. Molecular Systematics Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, Uttar Pradesh, India;2. Department of Biotechnology, Kumaun University, Bhimtal, Nainital 263 136, Uttarakhand, India;1. Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, China;2. Key Laboratory for Bio-Resources and Eco-Environment, College of Life Science, Sichuan University, Chengdu, Sichuan 610065, China;3. University of Chinese Academy of Sciences, Beijing 100049, China;1. Departamento de Ecología Evolutiva, Museo Nacional de Ciencias Naturales, CSIC, José Gutiérrez Abascal 2, 28006 Madrid, Spain;2. CRARC, Catalonian Reptile and Amphibian Recovery Center, 08783 Masquefa, Barcelona, Spain;3. Cabildo Insular de El Hierro, Centro de Reproducción e Investigación del lagarto gigante de El Hierro, El Hierro, Canary Islands, Spain;1. Department of Chemistry, Villanova University, Villanova, PA 19085, USA;2. Department of Food Science, Cornell University, Ithaca, NY 14853, USA;3. Department of Biodiversity Earth and Environmental Sciences, Drexel University, Philadelphia, PA 19104, USA;4. Department of Botany, Academy of Natural Sciences of Drexel University, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103, USA;1. School of Life Sciences, Sichuan Agricultural University, Yaan, Sichuan 625014, China;2. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;3. Alpine Crop Research Station, Xichang Institute of Agricultural Science, Liangshan Yi People Autonomous Prefecture, Xichang, Sichuan 616150, China;4. School of Resources and Environment, Sichuan Agricultural University, Chengdu, Sichuan 611130, China;5. Department of Food Science, Sichuan Tourism University, Chengdu, Sichuan 610100, China;1. Department of Pharmacy, University of Pisa, Via Bonanno 33, 56126 Pisa, Italy;2. Department of Environmental and Life Science, Section Botany and Botanical Garden, University of Cagliari, Viale S.Ignazio, 13 I, 09123 Cagliari, Italy;3. Institute of Life Sciences – Scuola Superiore Sant''Anna, Via Santa Cecilia 3, 56127 Pisa, Italy |
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| Abstract: | Genetic variability and population structure of Bergenia ciliata (Haw.) Sternb., commonly known as “Pashanbheda” (Stone-breaker), collected from the Western Himalayan region of India were estimated using two DNA fingerprinting methods viz., directed amplification of minisatellite DNA (DAMD) and inter simple sequence repeats (ISSR). The cumulative data analysis of DAMD and ISSR markers for 74 accessions from eight populations showed 86.1% polymorphism. Analysis of molecular variance (AMOVA) showed highest percentage of variation within individuals of populations (73.6%) and 21.7% among populations. STRUCTURE and PCoA analyses on the hierarchical partitioning of genetic diversity showed strong admixture of individuals among the eight assumed geographical populations of B. ciliata. The data suggests that high genetic flow is one of the major factors responsible for low genetic differentiation. Preservation of genetic diversity of B. ciliata is important, both to promote adaptability of the populations to changing environment as well as to preserve a large gene pool for future prospection. The present study using DAMD and ISSR markers, therefore, provide the means of rapid characterization of accessions within the populations, and thus enable the selection of appropriate accessions for further utilization in conservation and prospection programmes. |
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| Keywords: | DAMD Genetic diversity ISSR Population structure |
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