DNA barcoding in medicinal plants: Testing the potential of a proposed barcoding marker for identification of Uncaria species from China |
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| Institution: | 1. The Key Laboratory of Dai and Southern Medicine of Xishuangbanna Dai Autonomous Prefecture, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Jinghong 666100, China;2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;1. Dipartimento di Ecologia e Biologia (DEB), Università degli Studi della Tuscia, via S. Camillo de’ Lellis, 01100 Viterbo, Italy;2. Dipartimento di Agricoltura, Foreste, Natura ed Energia (DAFNE), Università degli Studi della Tuscia, via S. Camillo de’ Lellis, 01100 Viterbo, Italy;3. Stazione Zoologica Sperimentale Anton Dohrn, Villa Comunale, 80121 Napoli, Italy;1. Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, PR China;2. Guangzhou Baiyunshan Zhongyi Pharmaceutical Company Limited, Guangzhou 510530, PR China;1. The Graduate School, University of Santo Tomas, Espana, Manila 1015, Philippines;2. College of Science, University of Santo Tomas, Espana, Manila 1015, Philippines;3. Research Center for the Natural and Applied Sciences, University of Santo Tomas, Espana, Manila 1015, Philippines;1. Department of Biology, Long Island University-Brooklyn, 1 University Plaza, Brooklyn, NY 11201, United States;2. Center for Genomics and Systems Biology, New York University, 12 Waverly Place, New York, NY 10003, United States |
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| Abstract: | DNA barcoding, an increasingly popular mean of species identification, has been widely used for global species identification despite a consensus not being reached regarding which DNA sequences can be used as the best plant barcodes. In this study, we tested the feasibility of five candidate DNA barcodes (nrITS, nrITS2, matk, rbcL and trnH-psbA) for identifying Uncaria species. We collected a total of 54 specimens of 10 Uncaria species across its distributional range. BLAST, barcoding gaps, tree-based methods and TAXONDNA analysis were used to investigate the molecular identification capability of the candidate DNA barcodes. The results showed that the ITS2 is most suitable as a candidate DNA barcode for identification of medicinal plants of the genus Uncaria. |
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| Keywords: | DNA barcoding Internal transcribed spacer-2 (ITS2) |
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