Identification,development, and application of 12 polymorphic EST-SSR markers for an endemic Chinese walnut (Juglans cathayensis L.) using next-generation sequencing technology |
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| Institution: | 1. Laboratoire de Biochimie des Lipides, Département de Biologie, Université de Tunis El Manar, 2092 Tunis, Tunisia;2. Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), 41012 Sevilla, Spain;3. Institut National de Recherches en Génie Rural, Eaux et Forêts (INRGREF), Tunis, Tunisia;1. School of Biology and Basic Medical Sciences, Soochow University, 199, Renai Road, Suzhou, Jiangsu 215123, PR China;2. Department of Biology & School of Interdisciplinary Informatics, University of Nebraska at Omaha, Omaha, NE 68182-0040, United States;1. Key Laboratory for Forest Resource Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China;2. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, China;3. Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China;1. Shandong Institute of Sericulture, Shandong Academy of Agricultural Sciences, Yantai 264002, Shandong, PR China;2. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, Yunnan, PR China;3. Agriculture College, Ludong University, Yantai 264025, Shandong, PR China;4. Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR China;5. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China;6. College of Biological Science and Engineering, Beifang University of Nationalities, Yinchuan 750021, PR China;7. College of Resource and Environment, Yunnan Agricultural University, Kunming 650100, PR China;8. The New Zealand Institute for Plant and Food Research Limited, Christchurch 8140, New Zealand |
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| Abstract: | The Chinese walnut (Juglans cathayensis L.), valued for both its nut and wood, is an ecologically important tree species endemic temperate southern China. Investigation of the genetic diversity of Chinese walnut has been limited to natural population genetics and genetic germplasm resources. Here, we describe the development of 12 polymorphic microsatellite markers using next-generation sequencing to screen 96 Chinese walnut individuals collected from 11 natural populations. The number of alleles per locus ranged from 5 to 12. The observed heterozygosity (0.288–0.748) overlapped well with the expected heterozygosity (0.337–0.751). This species has high genetic diversity and gene flow among different populations (FST = 0.075, Nm = 3.088). These markers will be useful for future studies on population genetic structure, evolutionary ecology, and genetic breeding of this walnut tree or other Juglans species. |
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