Genetic diversity and population structure of Taxus cuspidata in the Changbai Mountains assessed by chloroplast DNA sequences and microsatellite markers |
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| Affiliation: | 1. College of Life Sciences, Shaanxi Normal University, Xi''an 710062, China;2. National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, Xi''an, Shaanxi 710062, China;3. Meteorological Bureau of Shaanxi Province, Xi''an 710014, China;1. Department of Biology Education, Seowon University, Cheongju 361-742, Republic of Korea;2. BioC, GReB, Botanic Institute of Barcelona (IBB-CSIC-ICUB), Passeig del Migdia s/n, Barcelona 08038, Spain;3. Department of Biology and the Research Institute of Natural Science, Gyeongsang National University, Jinju 660-701, Republic of Korea;1. Department of Agricultural, Food and Environmental Sciences, Polytechnic University of Marche, Via delle Brecce Bianche 10, I-60131 Ancona, Italy;2. Department of Natural Resources and Environmental Science, University of Nevada Reno, 1664 N. Virginia St., Reno, NV 89557, USA;3. State Forestry Service (CFS) Corps – Province Headquarter, Viale Croce Benedetto 47, I-63100 Ascoli Piceno, Italy;1. Corvinus University of Budapest, Department of Genetics and Plant Breeding, 1118 Budapest, Hungary;2. Agroscope, Institute for Plant Production Sciences IPS, 1964 Conthey, Switzerland;3. Corvinus University of Budapest, Department of Biometrics and Agrarinformatics, Hungary;1. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, 510520, China;2. Guangzhou Academy of Forestry and Landscape Gardening, Guangzhou, 510405, China |
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| Abstract: | Taxus cuspidata, a tree species with high economic value because of its anticancer properties, is experiencing severe reduction in populations across its range in China. We examined one chloroplast DNA (cpDNA) region (petA-psbE) and 9 nuclear simple sequence repeats (SSRs) loci variations among seven populations in the Changbai Mountains of China to investigate the levels of genetic diversity and population structure. A moderate level of haplotype diversity (HT = 0.625), low nuclear microsatellite diversity (HE = 0.261 ± 0.028), significant genetic differences (FST = 0.065) and substantial gene flow (Nm = 2.806) were observed. Most of the total genetic variation was partitioned within the population (87.8% and 94.0% for cpDNA and SSRs, respectively). Our haplotype identification permutation tests revealed that GST > NST, indicating an absence of phylogeographic structure in T. cuspidata. Neither STRUCTURE nor UPGMA analyses showed any geographic pattern in T. cuspidata populations. By comparatively analyzing the genetic diversity and survival situation of T. cuspidata, our results provide a theoretical foundation for the resource protection, utilization cultivation and breeding of this valuable plant. |
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| Keywords: | Changbai Mountains Genetic diversity Population structure Geographic pattern |
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